2000系列、3000系列、3200系列、4000系列、5000系列：打开机械泵电源，等待约1 h，打开质谱主机电源。

3500系列、4500系列、5500系列、6500系列：直接打开质谱主机电源。

激活质谱，打开软件右下角质谱图标，观察分子泵及真空读数状态，是否正常开启。抽真空过夜，观察真空读数，达到质谱要求真空度后才能正常使用。

4、待机真空数值

• 
  LCQ/LTQ系列离子阱液质联用仪 LCQ/LTQ series ion trap liquid mass spectrometer

关机步骤 Shutdown steps

1. 分析完成后，运行洗柱和保护柱的程序，洗柱完成后，将液相泵流速设置为0。1. After the analysis is completed, run the program of column washing and guard column. After the column washing is completed, set the liquid phase pump flow rate to 0.

2. 将质谱设置为待机Standby状态。 2. Set the mass spectrometer to standby state.

3. 关闭电子服务开关（Electronics Service Switch）。3. Turn off the Electronics Service Switch.

4. 关闭质谱仪主电源开关（Main Power Switch）至Off位置。4. Turn off the mass spectrometer’s Main Power Switch to the Off position.

5. 关闭液相部分电源开关。 5. Turn off the power switch of the liquid phase section.

6. 关闭计算机。 6. Shut down the computer.

7. 关闭UPS电源，关闭氮气和氦气钢瓶总阀及分压阀。7. Turn off the UPS power supply, and close the main valve and pressure dividing valve of nitrogen and helium cylinders.

开机步骤 Boot steps(从Off\停机至就绪\Standby状态)  (From Off\Stop to Ready\Standby state)

1. 打开氦气钢瓶的开关，确认总压大于2MPa，分压调至0.3Mpa，分压要求在200至350 kPa之间（30-50 psi）。1. Turn on the switch of the helium cylinder, confirm that the total pressure is greater than 2MPa, adjust the partial pressure to 0.3Mpa, and the partial pressure is required to be between 200 and 350 kPa (30-50 psi).

2. 打开氮气钢瓶的开关，确认总压大于2MPa，分压调至0.7MPa，分压要求在550至830 kPa（80-120 psi）之间。2. Turn on the switch of the nitrogen cylinder, confirm that the total pressure is greater than 2MPa, adjust the partial pressure to 0.7MPa, and the partial pressure is required to be between 550 and 830 kPa (80-120 psi).

3. 打开UPS开关，检查电源电压输出是否稳定且零地电压是否小于1V。3. Turn on the UPS switch and check whether the power supply voltage output is stable and whether the zero-ground voltage is less than 1V.

4. 检查机械泵，查看泵油颜色是否正常，呈浅黄色，油面在中间刻度线以上。打开仪器主电源开关（Main power）——On（I）模式，此时机械泵启动，分子涡轮泵也随之启动并逐渐增速。4. Check the mechanical pump to see if the color of the pump oil is normal, light yellow, and the oil level is above the middle mark. Turn on the instrument's main power switch (Main power) - On (I) mode. At this time, the mechanical pump starts, and the molecular turbo pump also starts and gradually increases its speed.

5. 打开主电源超过8小时后，打开计算机，将电子开关打开至Electronics Normal（I）模式。此时电子开关被打开，仪器前面板LED灯点亮、LCQ Fleet开始与计算机进行通讯（计算机上保持 instrument console 窗口不要关闭）。5. After turning on the main power for more than 8 hours, turn on the computer and turn the electronic switch to Electronics Normal (I) mode. At this time, the electronic switch is turned on, the LED light on the front panel of the instrument lights up, and LCQ Fleet begins to communicate with the computer (keep the instrument console window on the computer closed).

6. 约3分钟以后，LCQ Fleet与计算机通讯完成。6. After about 3 minutes, the communication between LCQ Fleet and the computer is completed.

7. 在Tune Plus界面右侧状态栏中，检查LCQ Fleet真空度情况。前级真空度（Convectron gauge）要求在1.2 ~ 1.8 Torr，离子规（Ion gauge）读数要求在0.75 × 10-5至1.5 × 10-5 Torr之间（离子传输毛细管开放，温度250 ℃）。7. In the status bar on the right side of the Tune Plus interface, check the vacuum level of LCQ Fleet. The front stage vacuum degree (Convectron gauge) is required to be between 1.2 ~ 1.8 Torr, and the ion gauge (Ion gauge) reading is required to be between 0.75 × 10-5 and 1.5 × 10-5 Torr (the ion transmission capillary is open and the temperature is 250°C).

8. 若真空满足要求，即可将质谱切至扫描（On）模式，校正仪器。8. If the vacuum meets the requirements, switch the mass spectrometer to scan (On) mode and calibrate the instrument.

9. 液相部分电源开启，更换流动相。 9. Turn on the power supply of the liquid phase section and replace the mobile phase.

10. 设定质谱及色谱条件参数，平衡色谱柱，准备进样。10. Set the mass spectrometry and chromatography condition parameters, equilibrate the chromatographic column, and prepare for injection.

• QE和OE系列高分辨液质联用仪 QE and OE series high-resolution LC/MS

关机步骤 Shutdown steps

1. 分析完成后，运行洗柱和保护柱的程序，洗柱完成后，将液相泵流速设置为0。1. After the analysis is completed, run the program of column washing and guard column. After the column washing is completed, set the liquid phase pump flow rate to 0.

2. 仪器standby。 2. Instrument standby.

3. 关闭电子服务开关（Electronics Service Switch）3. Turn off the Electronics Service Switch

4. 等2 min后关主开关（Main Power）。4. Wait 2 minutes and then turn off the main switch (Main Power).

5. 关液相部分。 5. Close the liquid phase section.

6. 关电脑。 6. Turn off the computer.

7. 关UPS。 7. Turn off the UPS.

8. 15 min后关高纯氮钢瓶总阀和分压阀以及氮气发生器。8. After 15 minutes, close the main valve, partial pressure valve and nitrogen generator of the high-purity nitrogen cylinder.

9. 倾倒废液。 9. Dump the waste liquid.

开机步骤 Boot steps

1. 打开氮气钢瓶的开关和氮气发生器，高纯氮0.6 MPa，氮气发生器7.1 bar左右，使气体充满管路并且稳定。1. Turn on the switch of the nitrogen cylinder and the nitrogen generator. The high purity nitrogen is 0.6 MPa and the nitrogen generator is about 7.1 bar, so that the gas fills the pipeline and is stable.

2. 打开UPS开关，检查电源电压输出是否稳定且零地电压是否小于1V。2. Turn on the UPS switch and check whether the power supply voltage output is stable and whether the zero-ground voltage is less than 1V.

3. 15 min后开质谱主开关（Main Power），抽真空1-2 h，开电子开关。3. After 15 minutes, turn on the main switch of the mass spectrometer (Main Power), evacuate for 1-2 hours, and turn on the electronic switch.

4. 开Tune软件，联机正常后，开启烘烤程序烘烤真空腔体：QE系列手动烘烤，OE系列自动烘烤。4. Open the Tune software. After the connection is normal, start the baking program to bake the vacuum chamber: manual baking for the QE series and automatic baking for the OE series.

5. 烘烤结束后，正常情况下，质谱面板指示灯为绿色，前级真空在1.5-2.0Torr之间，分子涡轮泵转速为750Hz，UHV小于5*e-8 Torr；开启Tune软件烘烤10-12h,次日UHV真空小于1.2*e-9 Torr。5. After baking, under normal circumstances, the indicator light on the mass spectrometer panel is green, the front vacuum is between 1.5-2.0Torr, the molecular turbo pump speed is 750Hz, and the UHV is less than 5*e-8 Torr; turn on the Tune software for baking 10-12h, the UHV vacuum is less than 1.2*e-9 Torr the next day.

6. 若真空满足要求，即可将质谱切至扫描（On）模式，校正仪器。6. If the vacuum meets the requirements, switch the mass spectrometer to scan (On) mode and calibrate the instrument.

7. 液相部分电源开启，更换流动相。 7. Turn on the power supply of the liquid phase section and replace the mobile phase.

8. 设定质谱及色谱条件参数，平衡色谱柱，准备进样。8. Set the mass spectrometry and chromatography condition parameters, equilibrate the chromatographic column, and prepare for injection.

• 
  Orbitrap Fusion系列高分辨液质联用仪Orbitrap Fusion Series High Resolution LC/MS

关机步骤 Shutdown steps

1. 分析完成后，将液相泵流速设置为0。 1. After the analysis is completed, set the liquid phase pump flow rate to 0.

2. 仪器standby。 2. Instrument standby.

3. 关闭电子服务开关（Electronics Service Switch）3. Turn off the Electronics Service Switch

4. 等2 min后关主开关（Main Power）。4. Wait 2 minutes and then turn off the main switch (Main Power).

5. 关液相部分。 5. Close the liquid phase section.

6. 关电脑。 6. Turn off the computer.

7. 关UPS。 7. Turn off the UPS.

8. 15 min后关高纯氮和高纯氦钢瓶总阀和分压阀。8. After 15 minutes, close the main valve and partial pressure valve of the high-purity nitrogen and high-purity helium cylinders.

9. 倾倒废液。 9. Dump the waste liquid.

开机步骤 Boot steps

1. 打开氮气和氦气钢瓶的开关，高纯氮50 ± 10 psi，高纯氦40 ± 10 psi，使气体充满管路并且稳定。1. Turn on the switches of nitrogen and helium cylinders, high-purity nitrogen 50 ± 10 psi, high-purity helium 40 ± 10 psi, so that the gas fills the pipeline and is stable.

2. 打开UPS开关，检查电源电压输出是否稳定且零地电压是否小于1V。2. Turn on the UPS switch and check whether the power supply voltage output is stable and whether the zero-ground voltage is less than 1V.

3. 15 min后开质谱主开关（Main Power），抽真空1-2 h，开电子开关。3. After 15 minutes, turn on the main switch of the mass spectrometer (Main Power), evacuate for 1-2 hours, and turn on the electronic switch.

4. 开Tune软件，联机正常后，开启烘烤程序烘烤真空腔体。4. Open the Tune software. After the connection is normal, start the baking program to bake the vacuum cavity.

5. 烘烤结束后，正常情况下，质谱面板指示灯为绿色，同时Tune软件参数均为绿灯，Orbitrap UHV < 2.5e-10 Torr。5. After baking, under normal circumstances, the indicator light on the mass spectrometer panel is green, and the Tune software parameters are all green. Orbitrap UHV < 2.5e-10 Torr.

6. 若真空满足要求，即可将质谱切至扫描（On）模式，校正仪器。6. If the vacuum meets the requirements, switch the mass spectrometer to scan (On) mode and calibrate the instrument.

7. 液相部分电源开启，更换流动相。 7. Turn on the power supply of the liquid phase section and replace the mobile phase.

8. 设定质谱及色谱条件参数，准备进样。 8. Set the mass spectrometry and chromatography condition parameters and prepare for injection.

• Trace ISQ单四级杆气质联用仪 Trace ISQ single quadrupole GC/MS

关机步骤 Shutdown steps

1. 在ISQ Dashboard中选择Instrument Control，先设定MS transfer line Temp 至50 °C，Ion source temp至50 °C，Send发送参数给仪器，后点击Shut down，等待Turbo‐pump Speed：0%，各温度需降至100 °C以下。1. Select Instrument Control in ISQ Dashboard, first set MS transfer line Temp to 50 °C, Ion source temp to 50 °C, Send parameters to the instrument, then click Shut down, wait for Turbo-pump Speed: 0%, Each temperature needs to be reduced to below 100 °C.

2. 在ISQ Dashboard中选择Method Editor/Trace1300 GC中Get Method form GC查看仪器当前参数设置，将S/SL Inlet Temperature勾选去除（如配置气相检测器需将检测器Temperature勾选去除），再选择Trace1300 菜单中的Send Method to GC 将现有参数发送给仪器，等待进样口温度降至100 °C以下。2. In the ISQ Dashboard, select Get Method form GC in Method Editor/Trace1300 GC to view the current parameter settings of the instrument, uncheck S/SL Inlet Temperature (if you configure a gas phase detector, you need to uncheck the detector Temperature), and then select Send Method to GC in the Trace1300 menu sends the existing parameters to the instrument and waits for the inlet temperature to drop below 100 °C.

3. 关闭ISQ的电源，关闭GC电源。 3. Turn off the power of the ISQ and turn off the power of the GC.

4. 关闭钢瓶总阀及分压阀。 4. Close the cylinder main valve and pressure dividing valve.

开机步骤 Boot steps

1. 确认GC进样口端和检测器端安装好毛细柱。 1. Confirm that the capillary columns are installed at the GC inlet and detector ends.

2. 确认ISQ的放空阀旋紧。 2. Make sure the ISQ vent valve is tightened.

3. 检查载气净化器是否失效，如已失效需更换新的。3. Check whether the carrier gas purifier has failed. If it has failed, replace it with a new one.

4. 如需更换机械泵泵油、进样针、隔垫及衬管，开机前进行更换。4. If you need to replace the mechanical pump oil, injection needle, septum and liner, do so before starting the machine.

5. 打开载气（氦气）钢瓶的开关，确认总压大于2 MPa，分压表调至0.5 MPa。5. Turn on the switch of the carrier gas (helium) cylinder, confirm that the total pressure is greater than 2 MPa, and adjust the partial pressure gauge to 0.5 MPa.

6. 确认插座，保证Trace 1300和ISQ已经接通电源。6. Check the socket to ensure that Trace 1300 and ISQ are powered on.

7. 打开电脑、气相、ISQ和自动进样器的电源开关，等待仪器自检。7. Turn on the power switches of the computer, gas phase, ISQ and automatic sampler, and wait for the instrument self-test.

8. 打开电脑桌面上工作站软件，连接仪器，检查软件通讯是否正常。8. Open the workstation software on the computer desktop, connect the instrument, and check whether the software communication is normal.

9. 找到对应设置柱信息的命令标签（Column Setup或者Column Properties），输入使用柱子的柱长内经等参数。9. Find the command label (Column Setup or Column Properties) corresponding to setting the column information, and enter the column length, inner diameter and other parameters of the column to be used.

10. 设置进样口的温度、载气和分流流量等参数，勾选“Vacuum compensation”。10. Set parameters such as inlet temperature, carrier gas, and split flow rate, and check "Vacuum compensation".

11. 在ISQ Dashboard 中查看ISQ状态，分子涡轮泵开始启动，待转速达到100%之后，在ISQ Dashboard中选择Instrument Control，设定MS transfer line Temp至250 °C，Ion source temp至280 °C，Send发送参数给仪器。11. Check the ISQ status in the ISQ Dashboard. The molecular turbo pump starts to start. After the rotation speed reaches 100%, select Instrument Control in the ISQ Dashboard and set the MS transfer line Temp to 250 °C and the Ion source temp to 280 °C. Send sends parameters to the instrument.

12. 建议抽真空过夜（前级泵压力≤ 80 mTorr），再进行调谐或使用仪器。12. It is recommended to evacuate overnight (foreline pump pressure ≤ 80 mTorr) before tuning or using the instrument.

• TSQ 8000三重四级杆气质联用仪 TSQ 8000 triple quadrupole GC-MS

关机步骤 Shutdown steps

1. 在TSQ Series Dashboard中选择Instrument Control，先设定MS transfer line Temp 至50 °C，Ion source temp至50°C，Send发送参数给仪器，后点击Shut down，等待Turbo‐pump Speed：0%，各温度需降至100 °C以下。1. Select Instrument Control in the TSQ Series Dashboard, first set the MS transfer line Temp to 50 °C, Ion source temp to 50°C, Send to send parameters to the instrument, then click Shut down, and wait for Turbo-pump Speed: 0% , each temperature needs to be reduced to below 100 °C.

2. 在TSQ Series Dashboard中选择Method Editor/Trace1300 GC中Get Method form GC查看仪器当前参数设置，将S/SL Inlet Temperature勾选去除（如配置气相检测器需将检测器Temperature勾选去除），再选择Trace1300菜单中的Send Method to GC将现有参数发送给仪器，等待进样口温度降至100 °C以下。2. In the TSQ Series Dashboard, select Get Method form GC in Method Editor/Trace1300 GC to view the current parameter settings of the instrument, uncheck S/SL Inlet Temperature (if configuring a gas phase detector, uncheck the detector Temperature), and then Select Send Method to GC in the Trace1300 menu to send the existing parameters to the instrument and wait for the inlet temperature to drop below 100 °C.

3. 关闭TSQ8000的电源，关闭GC的电源。 3. Turn off the power of the TSQ8000 and the GC.

4. 关闭钢瓶总阀及分压阀。 4. Close the cylinder main valve and pressure dividing valve.

开机步骤 Boot steps

1. 确认GC进样口端和MS端安装好毛细柱。 1. Confirm that the capillary columns are installed at the GC inlet end and MS end.

2. 确认TSQ8000的放空阀旋紧。 2. Confirm that the vent valve of TSQ8000 is tightened.

3. 检查载气净化器是否失效，如已失效需更换新的。3. Check whether the carrier gas purifier has failed. If it has failed, replace it with a new one.

4. 如需更换机械泵泵油、进样针、隔垫及衬管，开机前进行更换。4. If you need to replace the mechanical pump oil, injection needle, septum and liner, do so before starting the machine.

5. 打开载气（氦气）钢瓶的开关，确认总压大于2MPa，分压表调至0.5MPa。5. Turn on the switch of the carrier gas (helium) cylinder, confirm that the total pressure is greater than 2MPa, and adjust the partial pressure gauge to 0.5MPa.

6. 按照TSQ8000侧板上氩气接口上的压力数值设置分压表压力。6. Set the partial pressure gauge pressure according to the pressure value on the argon gas port on the side panel of TSQ8000.

7. 确认插座，保证Trace 1300和TSQ8000已经接通电源。7. Confirm the socket and ensure that Trace 1300 and TSQ8000 are powered on.

8. 打开电脑、气相、TSQ8000和自动进样器的电源开关，等待仪器自检。8. Turn on the power switches of the computer, gas phase, TSQ8000 and automatic sampler, and wait for the instrument self-test.

9. 打开电脑桌面上工作站软件，连接仪器，检查软件通讯是否正常。9. Open the workstation software on the computer desktop, connect the instrument, and check whether the software communication is normal.

10. 找到对应设置柱信息的命令标签（Column Setup或者 Column Properties），输入使用柱子的柱长内经等参数。10. Find the command label (Column Setup or Column Properties) corresponding to setting the column information, and enter the column length, inner diameter and other parameters of the column to be used.

11. 设置进样口的温度、载气和分流流量等参数，勾选“Vacuum compensation”。11. Set parameters such as the temperature, carrier gas and split flow rate of the inlet, and check "Vacuum compensation".

12. 在TSQ Series Dashboard中查看TSQ8000状态，分子涡轮泵开始启动，待转速达到100 %之后，前级泵压力达到200 mTorr以内，在TSQ Series Dashboard中选择Instrument Control，设定MS transfer line Temp 至250 °C，Ion source temp至280 °C，Send发送参数给仪器。12. Check the TSQ8000 status in the TSQ Series Dashboard. The molecular turbo pump starts to start. After the rotation speed reaches 100%, the backing pump pressure reaches within 200 mTorr. Select Instrument Control in the TSQ Series Dashboard and set the MS transfer line Temp to 250. °C, Ion source temp to 280 °C, Send sends parameters to the instrument.

13. 建议抽真空过夜（前级泵压力≤ 100 mTorr），再进行调谐或使用仪器。13. It is recommended to vacuum overnight (backing pump pressure ≤ 100 mTorr) before tuning or using the instrument.