使用三重串联四极杆液质联用系统开发了血清中 25-OH D2 和 25-OH D3 的快速定量分析法

使用三重串联四极杆液

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基于安捷伦 1200高分离快速液相色谱(RRLC)/组合离子源的 6430A三重串联四极杆液质联用系统开发了血清中 25-OH D2 和 25-OH D3 的快速定量分析法.

Abstract: 基于安捷伦 1200高分离快速液相色谱(RRLC)/组合离子源的 6430A三重串联四极杆液质联用系统开发了血清中 25-OH D2 和 25-OH D3 的快速定量分析法。固相萃取最大限度降低了基质干扰和离子抑制，同时还能自动化运行。在 5至 100 ng / mL的浓度范围内具有优异的定量线性度响应(R2 > 0.99)，而且分析时间只有五分钟，日间精密度远远低于 10%。血清中的检测限(LOD)和定量限(LOQ)分别是 2 ng/mL 和 3 ng/mL。 A rapid method for the quantification of 25-OH D2 and 25-OH D3 in serum was developed using the Agilent 1200 Series Rapid Resolution LC (RRLC) system coupled with an Agilent 6430 Triple Quadrupole LC/MS with multimode ionization (MMI). Solid phase extraction minimizes matrix interferences and ion suppression, while enabling automation. Excellent linearity of quantification (R2 > 0.99) was obtained in the range of 5 to 100 ng/mL, with run times of only 5 min, and day-to-day precision was well below 10%. The limits of detection (LOD) and limits of quantification (LOQ) were 2 ng/mL and 3 ng/mL respectively, in serum.

Keywords: 1200，快速高分离液相色谱，多模式进样口，血液，癌症，钙，磷 1200, RRLC, 6430, MMI, blood, cancer, calcium, phosphorus
Publication Number: 5990-4549CHCN
Last Updated: 9/3/2010
Number of Pages: 4

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内容来源：安捷伦

责任编辑：展源

审　　核：何发